Reporter

Part:BBa_K362060:Design

Designed by: Fumihiko Takeuchi, Aya Iwaki, Akiko Kawashima , Yuki Fukuyama   Group: iGEM10_KIT-Kyoto   (2010-10-25)


dps promoter - RBS - GFP - Term - Term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 534
    Illegal BamHI site found at 523
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1226


Design Notes

This composite part contains dps promoter(BBa_K362004),RBS(BBa_K121013),GFP(BBa_K121013) and 2 terminators on low copy vector(BBa_K121013). dps promoter has sensitibity to oxydative stress.



Source

dps promoter from E.coli genome

References